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yeast gal4 dna binding domain expression vector pas2 1  (TaKaRa)


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    Structured Review

    TaKaRa yeast gal4 dna binding domain expression vector pas2 1
    Yeast Gal4 Dna Binding Domain Expression Vector Pas2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+vector+pas2+1/pmCherry+Vector/pmc03695902-56-6-14
    Average 96 stars, based on 619 article reviews
    yeast gal4 dna binding domain expression vector pas2 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.
    Article Snippet: .. The truncated KIFC5A-s, amplified from mouse E13 hippocampal cDNA with oligo set 2, was transferred as a BamHI fragment into bacterial expression vector pHAT2 (Peränen et al., 1996) and from there as an EcoRI fragment into yeast vector pAS2.1 (Clontech). .. The full-length ORF of Nubp1, amplified from NIH 3T3 with oligo set 8, was initially transferred as an XhoI fragment into bacterial vector pCR2.1 (Invitrogen) or mammalian transfection vector pEGFP-C3 (BD Biosciences).

    Article Title: Protein Kinase A-anchoring Protein AKAP95 Interacts with MCM2, a Regulator of DNA Replication
    Article Snippet: .. Yeast Two-hybrid Screening—As bait, a full-length AKAP95 was amplified by PCR (9) and subcloned into yeast vector pAS2-1 (Clontech) to produce a Gal4BD-AKAP95 fusion protein. ..

    Article Title: Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.
    Article Snippet: From pCR2.1, it was subcloned in-frame as an EcoRI fragment in yeast vector pACT2 (Clontech). .. The cDNA of Nubp2, amplified from NIH 3T3 with oligo set 9, was initially transferred by T/A cloning into bacterial vector pCR2.1 (Invitrogen) and then subcloned as an EcoRI fragment into mammalian transfection vector pEGFP-N2 (BD Biosciences) and yeast vector pAS2.1 (Clontech). .. Nubp2 cDNA, amplified with oligo set 10 and transferred by T/A cloning into pCR2.1, was subcloned as a BamHI/XhoI fragment into bacterial vector pGEX-4T-1 (Amersham Biosciences) for expression of GST-tagged Nubp2 and to mammalian vector pSVmyc1.0 (Santama et al., 1998) for expression in NIH 3T3.

    Article Title: The KSHV immediate-early transcription factor RTA encodes ubiquitin E3 ligase activity that targets IRF7 for proteosome-mediated degradation.
    Article Snippet: .. The GAL4-RTA(1–544) DNA binding domain fusion protein in plasmidE6 (Scheffner et al., 1993). pYXY1 was generated in the yeast vector pAS2-1 (Clontech) by PCRTo begin to evaluate whether RTA may have a broader amplification from KSHV RTA cDNA.target specificity than just IRF7, we have also carried out preliminary experiments to test whether cotransfection with RTA negatively affects the accumulation of other Site-Directed Mutagenesis Several point mutants in RTA(1–691) plasmid pSEW-R01 were cre-IRFs and transcription factors. ..

    Expressing:

    Article Title: Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.
    Article Snippet: .. The truncated KIFC5A-s, amplified from mouse E13 hippocampal cDNA with oligo set 2, was transferred as a BamHI fragment into bacterial expression vector pHAT2 (Peränen et al., 1996) and from there as an EcoRI fragment into yeast vector pAS2.1 (Clontech). .. The full-length ORF of Nubp1, amplified from NIH 3T3 with oligo set 8, was initially transferred as an XhoI fragment into bacterial vector pCR2.1 (Invitrogen) or mammalian transfection vector pEGFP-C3 (BD Biosciences).

    Plasmid Preparation:

    Article Title: Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.
    Article Snippet: .. The truncated KIFC5A-s, amplified from mouse E13 hippocampal cDNA with oligo set 2, was transferred as a BamHI fragment into bacterial expression vector pHAT2 (Peränen et al., 1996) and from there as an EcoRI fragment into yeast vector pAS2.1 (Clontech). .. The full-length ORF of Nubp1, amplified from NIH 3T3 with oligo set 8, was initially transferred as an XhoI fragment into bacterial vector pCR2.1 (Invitrogen) or mammalian transfection vector pEGFP-C3 (BD Biosciences).

    Article Title: Protein Kinase A-anchoring Protein AKAP95 Interacts with MCM2, a Regulator of DNA Replication
    Article Snippet: .. Yeast Two-hybrid Screening—As bait, a full-length AKAP95 was amplified by PCR (9) and subcloned into yeast vector pAS2-1 (Clontech) to produce a Gal4BD-AKAP95 fusion protein. ..

    Article Title: The KSHV immediate-early transcription factor RTA encodes ubiquitin E3 ligase activity that targets IRF7 for proteosome-mediated degradation.
    Article Snippet: .. The GAL4-RTA(1–544) DNA binding domain fusion protein in plasmidE6 (Scheffner et al., 1993). pYXY1 was generated in the yeast vector pAS2-1 (Clontech) by PCRTo begin to evaluate whether RTA may have a broader amplification from KSHV RTA cDNA.target specificity than just IRF7, we have also carried out preliminary experiments to test whether cotransfection with RTA negatively affects the accumulation of other Site-Directed Mutagenesis Several point mutants in RTA(1–691) plasmid pSEW-R01 were cre-IRFs and transcription factors. ..

    Polymerase Chain Reaction:

    Article Title: Protein Kinase A-anchoring Protein AKAP95 Interacts with MCM2, a Regulator of DNA Replication
    Article Snippet: .. Yeast Two-hybrid Screening—As bait, a full-length AKAP95 was amplified by PCR (9) and subcloned into yeast vector pAS2-1 (Clontech) to produce a Gal4BD-AKAP95 fusion protein. ..

    Cloning:

    Article Title: Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.
    Article Snippet: From pCR2.1, it was subcloned in-frame as an EcoRI fragment in yeast vector pACT2 (Clontech). .. The cDNA of Nubp2, amplified from NIH 3T3 with oligo set 9, was initially transferred by T/A cloning into bacterial vector pCR2.1 (Invitrogen) and then subcloned as an EcoRI fragment into mammalian transfection vector pEGFP-N2 (BD Biosciences) and yeast vector pAS2.1 (Clontech). .. Nubp2 cDNA, amplified with oligo set 10 and transferred by T/A cloning into pCR2.1, was subcloned as a BamHI/XhoI fragment into bacterial vector pGEX-4T-1 (Amersham Biosciences) for expression of GST-tagged Nubp2 and to mammalian vector pSVmyc1.0 (Santama et al., 1998) for expression in NIH 3T3.

    Transfection:

    Article Title: Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.
    Article Snippet: From pCR2.1, it was subcloned in-frame as an EcoRI fragment in yeast vector pACT2 (Clontech). .. The cDNA of Nubp2, amplified from NIH 3T3 with oligo set 9, was initially transferred by T/A cloning into bacterial vector pCR2.1 (Invitrogen) and then subcloned as an EcoRI fragment into mammalian transfection vector pEGFP-N2 (BD Biosciences) and yeast vector pAS2.1 (Clontech). .. Nubp2 cDNA, amplified with oligo set 10 and transferred by T/A cloning into pCR2.1, was subcloned as a BamHI/XhoI fragment into bacterial vector pGEX-4T-1 (Amersham Biosciences) for expression of GST-tagged Nubp2 and to mammalian vector pSVmyc1.0 (Santama et al., 1998) for expression in NIH 3T3.

    Binding Assay:

    Article Title: The KSHV immediate-early transcription factor RTA encodes ubiquitin E3 ligase activity that targets IRF7 for proteosome-mediated degradation.
    Article Snippet: .. The GAL4-RTA(1–544) DNA binding domain fusion protein in plasmidE6 (Scheffner et al., 1993). pYXY1 was generated in the yeast vector pAS2-1 (Clontech) by PCRTo begin to evaluate whether RTA may have a broader amplification from KSHV RTA cDNA.target specificity than just IRF7, we have also carried out preliminary experiments to test whether cotransfection with RTA negatively affects the accumulation of other Site-Directed Mutagenesis Several point mutants in RTA(1–691) plasmid pSEW-R01 were cre-IRFs and transcription factors. ..

    Generated:

    Article Title: The KSHV immediate-early transcription factor RTA encodes ubiquitin E3 ligase activity that targets IRF7 for proteosome-mediated degradation.
    Article Snippet: .. The GAL4-RTA(1–544) DNA binding domain fusion protein in plasmidE6 (Scheffner et al., 1993). pYXY1 was generated in the yeast vector pAS2-1 (Clontech) by PCRTo begin to evaluate whether RTA may have a broader amplification from KSHV RTA cDNA.target specificity than just IRF7, we have also carried out preliminary experiments to test whether cotransfection with RTA negatively affects the accumulation of other Site-Directed Mutagenesis Several point mutants in RTA(1–691) plasmid pSEW-R01 were cre-IRFs and transcription factors. ..

    Cotransfection:

    Article Title: The KSHV immediate-early transcription factor RTA encodes ubiquitin E3 ligase activity that targets IRF7 for proteosome-mediated degradation.
    Article Snippet: .. The GAL4-RTA(1–544) DNA binding domain fusion protein in plasmidE6 (Scheffner et al., 1993). pYXY1 was generated in the yeast vector pAS2-1 (Clontech) by PCRTo begin to evaluate whether RTA may have a broader amplification from KSHV RTA cDNA.target specificity than just IRF7, we have also carried out preliminary experiments to test whether cotransfection with RTA negatively affects the accumulation of other Site-Directed Mutagenesis Several point mutants in RTA(1–691) plasmid pSEW-R01 were cre-IRFs and transcription factors. ..

    Mutagenesis:

    Article Title: The KSHV immediate-early transcription factor RTA encodes ubiquitin E3 ligase activity that targets IRF7 for proteosome-mediated degradation.
    Article Snippet: .. The GAL4-RTA(1–544) DNA binding domain fusion protein in plasmidE6 (Scheffner et al., 1993). pYXY1 was generated in the yeast vector pAS2-1 (Clontech) by PCRTo begin to evaluate whether RTA may have a broader amplification from KSHV RTA cDNA.target specificity than just IRF7, we have also carried out preliminary experiments to test whether cotransfection with RTA negatively affects the accumulation of other Site-Directed Mutagenesis Several point mutants in RTA(1–691) plasmid pSEW-R01 were cre-IRFs and transcription factors. ..



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